Synergists are typically non toxic chemical that are used to significantly to improve the efficacy of insecticides. They specifically inhibit the enzymes (such as cytochrome P450, carboxylesterases, glutathione s-transferases) that are responsible for the detoxification of insecticides, thereby making the exposed insects more susceptible. In many cases of insecticide resistance, the use f synergist have show an improvement of certain insecticides efficacy. The purpose of experiment is to used the synergist triphenyl phosphate (TPP) to inhibit carboxylesterases and to examine whether theses enzymes play any role in the detoxification of malathion in the lesser grain borer Rhyzopertha dominica (Coleoptera: Bostrichidae).
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