Insecticide bioassay is an important technique used to determine different toxicological parameters of an insecticide to which an given insect species or population has been exposed. It allows particularly the determination of LC50 and LD50 values and others parameters. There are several methods of insecticide exposure such as topical application and contact or residual bioassay. Among those two methods, topical application is the most accurate, however it is very time consuming. On the other hand, in residual exposure a known amount of the insecticide is dissolved in a volatile solvent such as acetone is added in scintillation vials. The acetone is evaporated and the insecticide coats the surface of the vial. Insect mortality data is recorded, and the data is analyzed using probit analysis where percent mortality is then converted to probit, and the dosage are transformed into log scale.
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